Confocal Raman microscopy is a high-resolution imaging technique that is widely used for the characterization of materials and specimens in terms of their chemical composition. With 2D and 3D Raman images, information regarding the chemical compounds and their distribution within the sample can be illustrated clearly.
The WITec Raman microscopes and imaging systems combine an extremely sensitive confocal microscope with an ultrahigh-throughput spectroscopy system for unprecedented chemical sensitivity. It facilitates, in combination with an excellent depth resolution and a strongly reduced background signal, the generation of depth profiles and 3D images with exceptional spectral and spatial resolution. This outstanding performance in speed, sensitivity and resolution can be jointly applied without compromises.
In Confocal Raman Imaging chemical properties of solid and liquid components can be analyzed with diffraction-limited spatial resolution (?/2 of the excitation wavelength, down to ca. 200 nm). No labeling or other sample preparation techniques are necessary.
The Raman technique
The Raman effect is based on light interacting with the chemical bonds of a sample. Due to vibrations in the chemical bonds this interaction causes a specific energy/frequency shift in the back scattered light which appear in a Raman spectrum. The Raman spectrum is unique for each chemical composition and can provide qualitative and quantitative information of the material. For Raman spectroscopy the Raman spectra are analyzed with a spectrometer.
The WITec apyron imaging system has been developed to overcome the boundary between ease-of-use and ultimate capability in Raman Imaging. It is ideal for multi-user/industry labs, Raman newcomers with advanced imaging requirements and established Raman spectroscopists.
apyron sets the benchmark for automated Raman Imaging systems offering: outstanding spectral and spatial resolution, ultra-fast acquisition times, and exceptional signal sensitivity in combination with automated system configurations and intuitive measurement procedures.
The alpha300 M is a high-quality yet affordable micro-Raman system that provides the same outstanding performance and spectral resolution that other WITec products are renowned for. This unique combination makes the alpha300 M the preferred instrument for non-destructive characterization and reliable Raman spectral analysis in many fields of application.
The well-established Raman-atomic force microscope (AFM) combination alpha300 RA was the first integrated Raman AFM system on the market and continues to set the standard for combined instrument configurations. The alpha300 RA incorporates the features of the Raman microscopy system alpha300 R for powerful chemical imaging along with features of the atomic force microscopy system (alpha300 A) for high-resolution nanoscale surface characterization and thus facilitates a comprehensive understanding of the samples.
The unprecedented all-in-one alpha300 RAS combines Raman, AFM, and SNOM imaging in a single instrument for the utmost flexibility and sophisticated sample characterization. By combining the imaging techniques, simultaneous Raman-AFM analysis, TERS, and Nearfield-Raman (Raman-SNOM) imaging can be easily performed.
The imaging capabilities of the alpha300 R fulfill the highest requirements of confocal Raman imaging with superior performance in speed, sensitivity and resolution. These unique characteristics have established the alpha300 R the preeminent confocal Raman imaging system on the market.
WITec´s award winning TrueSurface Microscopy allows confocal Raman imaging along heavily inclined or very rough samples with the surface held in constant focus while maintaining the highest confocality. The core element of this revolutionary imaging mode is an integrated sensor for optical profilometry. It is directly installed at the microscope turret which facilitates user-friendly and convenient handling.